Contribution of Flt3L-induced cDCs and pDCs to the induction of donor-specific central and peripheral tolerance. Enriched FL-cDCs and FL-pDCs from BALB/c mice were IV transferred 10 days after DC infusion, and alloreactivity of T cells from the recipient mice was assayed by MLR. (A) RNA was extracted from each DC subset to analyze the expression of various cytokines by RT-PCR. (B-C) MLR to B6, BALB/c, and C3H spleen APCs by recipient T cells prepared from (B) spleen and (C) mature thymocytes. Data in all panels are representative of at least 3 independent experiments.