Colocalization of p53 and CD71 in 5q− and DBA patient samples. Histologic sections of bone marrow biopsies from patients were analyzed by double immunofluorescence for p53 and cell surface markers (CD71 or GlyA). The antibodies used are indicated in each panel. Bone marrow biopsies are shown from patients with hemolytic anemia (A-B), del(5q) MDS (C-D), and DBA (E-F). (A-F) Original magnification ×400. The stained slides were scanned using the TissueFAXS Plus automated microscopic workstation (Tissuegnostics) with a Zeiss Axiolmager Z1 using a 40× Zeiss objective using a high sensitivity digital monochrome camera for fluorescence microscopy (TissueGnostics), and captured using TissueFAXS 1.3 software (TissueGnostics). Final images were transferred into Adobe Photoshop 6.0 where TIF files were created. Scale bar represents 25 μm.