Immune barriers in AKR/J → BALB.K mice. Recipient mice were given sublethal TBI and injected with anti-CD4 (GK1.5), anti-CD8 (53-6.7), anti-asialoGM1, or control IgG before infusion of donor HSCs. Shown are the percentages of mice with donor engraftment and chimerism characteristics at 6 weeks after transplantation. T-cell chimerism was evaluated by FACS for Thy1 surface markers. B-cell and myeloid chimerism was evaluated by PCR for D6Mit3 DNA markers of FACS-sorted B220⁺ and Mac1/Gr1⁺ blood cells.