RUC-1 inhibits aggregation of human platelets and hαIIb/mβ3 murine platelets, but not WT mouse or rat platelets. PRP was isolated from whole blood of WT Sprague-Dawley rats, WT C57Bl/6 mice, healthy human volunteers, or hybrid hαIIb/mβ3 mice, and was treated with either vehicle control or 100 μM RUC-1 for 5 minutes. Aggregation was initiated with ADP and light transmission was monitored through aggregometer cuvettes for 8 minutes. One representative plot from each animal is shown.