The full-length p53TAD binds to the zinc-finger domains of GATA-1 in an in vitro pulldown assay. (A) Schematic of the TAD of p53 demonstrating position of the full-length p53TAD and the location of the 2 independent subdomains: p53TAD1 and p53TAD2. (B) Binding of p53TAD and p53TAD1 to GST-GATA-1 ZFD (GATA-1_199-317). Various concentrations (1.0, 0.1, and 0.01 μM) of p53TAD (lanes 5-7) and p53TAD1 (lanes 8-10) were incubated with 1 μM GST-GATA-1 ZFD. In the GST lanes, 1 μM purified p53TAD (lane 3) and p53TAD1 (lane 4) was incubated with 1 μM GST as a control. The input lanes are p53TAD (lane 1) and p53TAD1 (lane 2) at 0.5%. Bound protein was detected with an anti-p53 antibody DO-1. (C) Binding of p53TAD and p53TAD2 to GATA-1 ZFD. Various concentrations (1.0, 0.1, and 0.01 μM) of p53TAD (lanes 5-7) and p53TAD2 (lanes 8-10) were incubated with 1 μM GST-GATA-1 ZFD. In the GST lanes, 1 μM purified p53TAD (lane 3) and p53TAD2 (lane 4) was incubated with 1 μM GST as a control. The input lanes are p53TAD (lane 1) and p53TAD2 (lane 2) at 0.5%. Bound protein was detected with an anti-p53 antibody Pab 1801.