Multiple sequence alignment of HJV. Alignment of regions of the human hemojuvelin protein sequence with the mutations, against sequences of the mouse, rat, and zebrafish hemojuvelin, and human, mouse, and zebrafish RGMs. Sequence alignment between the different species shows high sequence similarity. Leucine 194 is only found in mammalian HJV. Mutations are indicated by the arrows. * indicates identical residues in the alignment; colon (:), conserved substitutions; and period (.), semiconserved substitutions of residues. Alignments were performed with ClustalW version 2.0.10 (European Bioinformatics Institute, Cambridge, United Kingdom).