Thymocyte recovery after hydrocortisone treatment in Gli2ΔN2 and Gli2ΔC2 transgenic mice. (A) Dot plots show anti-CD4 and anti-CD8 staining for Gli2ΔN2 transgenic thymocytes and WT littermate thymocytes 2 to 6 days after treatment with HC. Bar chart shows the mean relative proportion of DP cells 5 days after HC treatment for WT and Gli2ΔN2 transgenic mice. The difference in mean percentage of DP cells was statistically significant (P = .01). (B) Dot plots show anti-CD4 and anti-CD8 staining for Gli2ΔC2 transgenic thymocytes and WT littermate thymocytes 2 to 5 days after HC treatment. Bar chart shows the mean relative proportion of DP cells 4 days after HC treatment. The difference in DP percentage was statistically significant (P = .004). (C) Graph of relative kinetics of DP recovery after HC treatment in WT, Gli2ΔN2, and Gli2ΔC2. Error bars represent SD. (Day 2: WT, n = 6; Gli2ΔN2, n = 3; Gli2ΔC2, n = 3. Day 4: WT, n = 7; Gli2ΔN2, n = 3; Gli2ΔC2, n = 3. Day 5: WT, n = 7; Gli2ΔN2, n = 3; Gli2ΔC2, n = 3. Day 6: WT, n = 5; Gli2ΔN2, n = 3; Gli2ΔC2, n = 2.)
