Plasma VWF multimers. (A) VWF multimer patterns. Plasma samples (1 μL/lane) from Adamts13^L/L, Adamts13^S/S, and Adamts13^−/− mice were electrophoresed on SDS-agarose gels and transferred to nitrocellulose membranes. VWF multimers were detected with anti-VWF antibodies. (B) Relative intensities of plasma VWF multimers. The chemiluminescent intensities of the VWF multimer patterns (A) were scanned using image analysis software. An average of multiple lanes from 4 mice for each genotype is shown. HMW indicates high molecular weight; LMW, low molecular weight.