Flow chart showing genealogy of cell lines and their titer output after induction and/or transient transfection. Transiently transfected components are indicated by brackets (see “Methods” for plasmid designations). Cell line names are in bold, and viral transductions with constructs diagrammed in Figure 1 are indicated on the left. Unconcentrated supernatant titers, in HeLa TU/mL, are shown on the right.