Relative gene expression of Mll5 in various FACS-sorted bone marrow and splenocyte subpopulations. Bone marrow (BM) and spleen (Spl) of 8-week-old wild-type mice were harvested and stained for FACSorting (see “FACS analysis/cell sorting, cell-cycle analysis, and apoptosis measurement”). Quantitative RT-PCR was performed in quadruplicate for Mll5 and Abl. The chart shows mean and standard deviation (SD) of expression levels of Mll5 mRNA relative to Abl normalized to one of the LSKFLT3neg samples.