The effects of SB203580, wortmannin, and LiCl on fucoidan- or anti–SR-A Ab–induced cytokine production and CD83 expression in PBDCs. (A) PBDCs were pretreated with SB203580 (5 μM), wortmannin (1 μM), or LiCl (10 mM) for 1 hour and then further cultured in the presence or absence of fucoidan, LPS, or TNF-α. After 24 hours, cytokine secretion was measured by ELISA. (B) In the top panel, PBDCs were pretreated with wortmannin (1 μM) for 1 hour and then further cultured in the presence or absence of anti–SR-A Ab (5 μg) or control Ab (5 μg). After 24 hours, mDC, pDC, and CD11c⁻CD123⁻ cells were gated and examined for CD83 expression. (Bottom panel) PBDCs were pretreated with kinase inhibitors as in panel A. The results represent the expression levels of CD83 on CD11c^highCD123^low mDCs. (C) The sorted CD11c⁺ mDCs were pretreated with kinase inhibitors as in panel A for 1 hour, after which they were further cultured with anti–SR-A Ab or control Ab for 24 hours. The results shown represent the mean ± SD of 3 independent experiments. *P < .05 compared with none; n.d. indicates not determined.