Stabilization of mutant α globins by AHSP or β globin in E coli. (A) The pETDuet-1 vector was used to coexpress α globin and AHSP or β globin from a single plasmid in E coli. This vector contains 2 multiple cloning sites, each of which is preceded by an IPTG-inducible T7 promoter/lac operator and a ribosome-binding site (rbs), followed by a single T7 terminator. The plasmids were transformed into E coli, and IPTG was used to induce protein expression. The cells were lysed, cytosolic fractions were separated by SDS-PAGE, and protein expression was measured by Western blotting with the appropriate antibodies. (B) Western blots showing coexpression of AHSP and various α globin mutant proteins in E coli. (C) Western blots showing coexpression of WT β globin and various α globin mutant proteins in E coli. Expressed β globin was detected using an antibody against HbA.