American Society of Hematology

Figure 2

$Figure 2. In vitro interactions of WT and mutant α globins with AHSP-GST. 35S-radiolabeled mutant α globins were synthesized by transcription coupled translation (TNT) with CN-hemin present, incubated with GST or GST-AHSP Sepharose beads, fractionated by SDS-PAGE, and visualized by autoradiography. Input controls (not incubated with beads) are shown in the top panel.$

In vitro interactions of WT and mutant α globins with AHSP-GST. ³⁵S-radiolabeled mutant α globins were synthesized by transcription coupled translation (TNT) with CN-hemin present, incubated with GST or GST-AHSP Sepharose beads, fractionated by SDS-PAGE, and visualized by autoradiography. Input controls (not incubated with beads) are shown in the top panel.

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