Enzastaurin triggers β-catenin accumulation in MM and other tumor cells by inhibiting its degradation. (A-C) Indicated cell lines were treated with enzastaurin for 4 hours and subjected either to cell lysis (A,C) followed by immunoblotting with indicated antibodies or RNA extraction (B) followed by specific quantitative real-time PCR for β-catenin. (D) MM.1S cells were exposed to the indicated concentrations of enzastaurin for 4 hours followed by cell fractionation. Immunoblots were probed with the indicated antibodies.