Inhibition of EPC adhesion by VEGI. (A) The ability of EPCs cultured for 7 days in the absence or presence VEGI to adhere on surfaces coated with various extracellular matrix proteins, as indicated. (B) Confocal microscopic images of immunofluorescent staining for integrins on EPCs cultured for 10 days in the absence or presence of VEGI. Green indicates integrin α5; red, integrin αv; blue, cell nuclei (60×60 objective lens). (C) Intensities of immunofluorescent staining of integrins α5 and αv. □ represents untreated; ■, VEGI-treated. (D) Intensities of immunofluorescent staining for cell signaling molecules involved in adhesion. EPCs cultured for 10 days in the absence or presence of VEGI were labeled for ILK, phospho-FAK, phospho-paxillin, or phospho-Src. □ represents untreated; ■, VEGI-treated. Values are mean ± SE of 3 independent experiments. *P < .05.