Intracellular distribution of uPA compared with α_IIbβ₃, and α-granule proteins TSP-1 and MMRN1 in day-13 QPD and control cultured megakaryocytes. Panels show deconvolved immunofluorescent images of megakaryocytes, double immunolabeled with rabbit anti-uPA (red, left panels) and mouse monoclonal antibodies to α_IIbβ₃, TSP-1, or MMRN1 (green, middle panels) (merged images on right). Insets show magnified images of peripheral cytoplasm. Experiments were performed as outlined in “Immunofluorescent microscopy.”