Immunization schedule and plasmid maps. (A) Mice were immunized on day 0 and day 14 with a multiclade HIV Gag construct and with or without adjuvant, followed by electroporation using the CELLECTRA adaptive constant current device after each immunization. On day 21, mice were killed and lymphocytes were isolated and analyzed. (B) Plasmid maps for murine IL-28B and IL-12 constructs.