Figure 3
Characterization of rare Blimp1gfp/gfp tumors. (A) Representative histologic appearance of tumors by Blimp1 genotype. (Top) PCT in a Blimp1gfp/+ mouse, characteristic of tumors seen in all Blimp1+/+ and Blimp1gfp/+ mice. (Middle) Reticulum cell sarcoma, as seen in 4 Blimp1gfp/gfp mice. (Bottom) Lymphoid leukemia in Blimp1gfp/gfp mouse no. 28. Images were viewed using a Zeiss Axiophot microscope (Oberkochen, Germany) with a Plan-apo 20×/0.60 objective and hematoxylin and eosin stain and captured with a Zeiss Axiophot camera. (B) Flow cytometric analysis of BM, spleens, and enlarged LN (“tumors”) of 2 representative Blimp1gfp/gfp mice that developed tumors. The tissues of mouse no. 21 had a pattern characteristic of mice that bore reticulum cell sarcomas (nos. 17, 21, and 22). Mouse no. 28 had a lymphoid leukemia. The panel on the right for mouse no. 28 is gated on GFP+ cells in the tumor. (C) Quantitative reverse transcription PCR measuring expression levels of genes in normal resting B cells, GFP− B-cell blasts, GFP+ plasmablasts generated in vitro (27) and GFP+ cells sorted from 4 Blimp1gfp/+ and all 5 Blimp1gfp/gfp tumors. Note that the Blimp1 PCR amplifies exons that are not affected by the Blimp1/GFP modification, so both Blimp1+ and Blimp1gfp alleles are detected.

Characterization of rare Blimp1gfp/gfp tumors. (A) Representative histologic appearance of tumors by Blimp1 genotype. (Top) PCT in a Blimp1gfp/+ mouse, characteristic of tumors seen in all Blimp1+/+ and Blimp1gfp/+ mice. (Middle) Reticulum cell sarcoma, as seen in 4 Blimp1gfp/gfp mice. (Bottom) Lymphoid leukemia in Blimp1gfp/gfp mouse no. 28. Images were viewed using a Zeiss Axiophot microscope (Oberkochen, Germany) with a Plan-apo 20×/0.60 objective and hematoxylin and eosin stain and captured with a Zeiss Axiophot camera. (B) Flow cytometric analysis of BM, spleens, and enlarged LN (“tumors”) of 2 representative Blimp1gfp/gfp mice that developed tumors. The tissues of mouse no. 21 had a pattern characteristic of mice that bore reticulum cell sarcomas (nos. 17, 21, and 22). Mouse no. 28 had a lymphoid leukemia. The panel on the right for mouse no. 28 is gated on GFP+ cells in the tumor. (C) Quantitative reverse transcription PCR measuring expression levels of genes in normal resting B cells, GFP B-cell blasts, GFP+ plasmablasts generated in vitro (27 ) and GFP+ cells sorted from 4 Blimp1gfp/+ and all 5 Blimp1gfp/gfp tumors. Note that the Blimp1 PCR amplifies exons that are not affected by the Blimp1/GFP modification, so both Blimp1+ and Blimp1gfp alleles are detected.

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