Figure 3
Figure 3. Transgenic expression of constitutively active STAT5 accelerates pre–B-cell leukemogenesis in BLNK+/− mice. (A) Flow cytometric analysis of nucleated cells of spleens (panels in top 2 rows) and bone marrows (panels in bottom 2 rows) from mice of the indicated genotypes. Numbers indicate percentage of cells falling in each window. Data are representative of 3 independent experiments. (B) Kaplan-Meier curves depicting the proportion of mice that remain alive with age. BLNK+/− (○), STAT5b-CA (▵), and BLNK+/−STAT5b-CA (■) mice are shown. (C) Western blot analysis for BLNK and Btk (top) or β-actin (bottom) proteins in primary pre-B leukemia cells from individual lymph nodes (LN#) in 2 BLNK+/−STAT5b-CA mice (#1 and #2; top and bottom, respectively) or a STAT5b-CA mouse (top). As controls, proteins from MACS-purified spleen B cells from unaffected BLNK+/− (top) or STAT5b-CA (bottom) mouse are included.

Transgenic expression of constitutively active STAT5 accelerates pre–B-cell leukemogenesis in BLNK+/− mice. (A) Flow cytometric analysis of nucleated cells of spleens (panels in top 2 rows) and bone marrows (panels in bottom 2 rows) from mice of the indicated genotypes. Numbers indicate percentage of cells falling in each window. Data are representative of 3 independent experiments. (B) Kaplan-Meier curves depicting the proportion of mice that remain alive with age. BLNK+/− (○), STAT5b-CA (▵), and BLNK+/−STAT5b-CA (■) mice are shown. (C) Western blot analysis for BLNK and Btk (top) or β-actin (bottom) proteins in primary pre-B leukemia cells from individual lymph nodes (LN#) in 2 BLNK+/−STAT5b-CA mice (#1 and #2; top and bottom, respectively) or a STAT5b-CA mouse (top). As controls, proteins from MACS-purified spleen B cells from unaffected BLNK+/− (top) or STAT5b-CA (bottom) mouse are included.

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