Gene expression in Mll5^−/− HSC. (A) Genome-wide analysis of gene expression in Mll5^−/− and wild-type LSK cells. The plot shows normalized (log₂) hybridization signals for individual features on the microarrays probed with Mll5^−/− or wild-type labeled cRNA. The data were generated by using 4 independent preparations of RNA from flow-sorted LSK cells (2 preparations from Mll5^−/− mice and 2 from littermate controls). In each case, the LSK cells were prepared from bone marrow pooled from 6 mice. (B) Hox gene expression in Mll5^−/− LSK cells as determined by the microarray analysis shown in panel A. The graph shows means plus or minus SEM. (C) Quantitative RT-PCR analysis of Hoxb2 and Hoxb5 expression in Mll5^−/− LSK cells. Levels of expression were determined in triplicate and normalized to that of β-actin. The graph shows means plus or minus SD for samples from 1 pair of mice. The data are representative of 3 independent experiments.