Measurement of in vitro migration and F-actin polymerization of MPD and Gmob HCMCs. SCF (A) or IL-8 (B) induced migration of HCMCs from MPD patients (n = 21) and Gmob volunteers (n = 6). Each column represents the mean ± SD of the percentage of HCMCs from an individual PV, PMF, or Gmob volunteer, which have migrated. (C) F-actin contents in MPD (n = 5) or normal (n = 2) HCMCs. HCMCs were stimulated with 10 ng/mL SCF and fixed at the time points indicated by addition of formaldehyde. Results were expressed as mean channel fluorescence (MCF).