Measurement of the release of tryptase by MPD HCMCs. HCMCs from 9 MPD patients and 6 Gmob volunteers were stimulated with calcium ionophore to induce tryptase release for 60 minutes at either 37°C or 42°C. The levels of tryptase in the supernatants and cell pellets were measured by ELISA. (A) Each column represents the mean ± SD of the total tryptase in each group of HCMCs. (B) Each triangle represents the level of tryptase in the supernatant of HCMCs stimulated with calcium ionophore at the temperature indicated. Each bar represents the mean of each sample group.