The levels of histamine, LTs, and PGD₂ release by MPD HCMCs. HCMCs from 11 MPD patients and 6 Gmob volunteers were sensitized with IgE overnight and then were challenged with or without anti-IgE for 30 minutes at either 37°C or 42°C (10⁵/mL). The levels of histamine (A,D), PGD₂ (B,E), and LTs (C,F) in the supernatants were measured by ELISA. Each triangle represents the level of histamine in the supernatants of HCMCs sensitized with IgE (A-C) or HCMCs stimulated with IgE plus anti-IgE (D-F) at the temperature indicated. Each bar represents the mean of each sample group.