Figure 6
![Figure 6. Surface-exposed PfEMP1 is reduced in Pf332 KO parasite lines. Western blot analysis of Triton X-100-insoluble/SDS-soluble membrane fractions from uninfected RBCs or iRBCs (3D7, 3D7ΔPf332 [11G7, 12F3, 13E2, and 13F7]), either with (+) or without (−) treatment with trypsin. The 2 bands below full-length PfEMP1 at approximately 250 kDa represent cross-reactivity with the RBC cytoskeletal proteins α and β spectrin. The approximately 80-kDa band detected after treatment with trypsin (VARC) represents the cytoplasmic domain of surface-exposed PfEMP1 in these iRBCs. An identical blot was probed for HSP70 as a parasite-loading control and with anti–glycophorin C (GpC) as a control for digestion of RBC surface proteins.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/113/4/10.1182_blood-2008-05-157735/4/zh80240828310006.jpeg?Expires=1723246344&Signature=TFCUiocLH~GGJFUAVgp~BWhalAdevQpzwQ9-I11y5KBJ4h7hINMOn9znxmEq3V1gdxKuHZzyy6aONhpD7Vjq7FBYI3lDf7u9VGtQqiwOxB1lNF3Sg2A58R8vtoG1zzb8HhlayLOYUbVQha7cDoDD8Od6FcHM3iy2tM9B-F~y46c4GBZLoAWK6k9FKRb0BtumGUNXS9AEQWFIYvQfQQIWfQ6yetja36fBCjOGN4aufvnv889Uhc6yPCyX0xnTpS0Wam-obljgK8-zqgtJBekVnvlGiPcWJQsbLQtj~oK-VV6I6LiLYgqvfQzLFmzC8DmMdfEjrzYvqtNe-u8Ll5ckxA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Surface-exposed PfEMP1 is reduced in Pf332 KO parasite lines. Western blot analysis of Triton X-100-insoluble/SDS-soluble membrane fractions from uninfected RBCs or iRBCs (3D7, 3D7ΔPf332 [11G7, 12F3, 13E2, and 13F7]), either with (+) or without (−) treatment with trypsin. The 2 bands below full-length PfEMP1 at approximately 250 kDa represent cross-reactivity with the RBC cytoskeletal proteins α and β spectrin. The approximately 80-kDa band detected after treatment with trypsin (VARC) represents the cytoplasmic domain of surface-exposed PfEMP1 in these iRBCs. An identical blot was probed for HSP70 as a parasite-loading control and with anti–glycophorin C (GpC) as a control for digestion of RBC surface proteins.
