Disruption of Pf332 reduces CD36-mediated adhesion of iRBCs to human platelets under flow and static conditions. Level of adhesion of iRBCs to purified or cell-expressed CD36 from KO 3D7ΔPf332 (11G7, 12F3, 13E2, and 13F7), truncation 3D7ΔPf332a (3F3 and 12H9), and truncation 3D7ΔPf332b (5G1 and 13F5) cultures measured under flow or static conditions. Adhesion represents the number of iRBCs that adhered for the transgenic parasite clones as a percentage of wild-type 3D7 iRBCs. In all assays, adhesion of noninfected RBCs was rare (< 0.05% of total adherent RBCs). Data represent the mean plus or minus SEM (error bars) for at least 3 independent experiments for each parasite clone.