Validation of the methylation array. (A) Methylation profiles of replicate samples show high reproducibility. Data shown are methylation β-values of 1505 CpG sites in which 1 and 0 represent high and low methylation levels, respectively. Each dot represents a CpG site. (B) Methylation array data correlate with methylation-enriched PCR results. Methylation-enriched PCR was used to measure the methylation status of CpG sites of DBC1 and DCC genes in bone marrow mononuclear cells from 3 healthy control donors and 5 patients with AML. ■ indicates the methylation-enriched PCR results represented as fold change compared with the results in the patient with AML marked with an asterisk. indicates the array average methylation values (β-value) for the same CpG sites.