S36578 acts through a MAP-dependent signaling pathway. (A) Western blot of ERK1/2 in HeLa cells and MDMs treated with S36578. Treatment at 4 and 24 hours is shown. Macrophage samples of 4 and 24 hours are from different donors. (B) Increased phosphorylation of p38-MAPK and HSP27 measured with human mercator phosphoarray. MDMs from 2 different donors (Donor 1 and Donor 2) and HeLa cells were analyzed, each of them at 2 different concentrations of S36578 (25 μM and 2.5 μM for MDMs; 2.5 μM and 0.3 μM for HeLa). The bar plots represent the averaged fluorescence intensity and SD for each sample. ND indicates no drug. (C) Western blot showing an increased phosphorylation of p38-MAPK in HeLa cells as a consequence of S36578 treatment.