Cytohesin-1 is up-regulated in human Mo-DCs during maturation, and is efficiently silenced by RNAi in both human and murine DCs. Western blot analysis shows that cytohesin-1 expression is up-regulated in human monocyte-derived dendritic cells (Mo-DCs) upon maturation induced by LPS (1 μg/mL)/TNF alpha (50 ng/mL; panel A lanes d8, d9, d10). Expression of cytohesin-1 is barely detectable in immature dendritic cells (imDCs), but maturation results in a strong increase of cytohesin-1 expression, reaching maximum levels at day 9 (A). Using square wave electroporation, cytohesin-1 expression was efficiently silenced in mature DCs. Western blot analysis shows that RNAi of cytohesin-1 reduces specific protein expression 96 hours after electroporation in human Mo-DCs (B) or murine bone marrow-derived DCs (BM-DCs, C).