Multiple DNA sequence alignment of RhAG proteins. A total of 37 RhAG and RhAG-like protein sequences were aligned using EMBL-EBI CLUSTALW (EMBL-EBI, Cambridge, United Kingdom) from sequences identified using the National Center for Biotechnology Information (NCBI, Bethesda, MD) Human Genome Resources and BLASTP. The species' name, protein number, and a portion of the sequence are shown (amino acids 59-102 of the human sequence). Amino acids 61 and 65 (of the human sequence) are boxed, showing that Ile61 is conserved and Phe65 is identical across all 37 sequences. This figure shows only a fragment of the RhAG protein sequence; a complete alignment of 111 Rh sequences can be found in the supporting information of Huang and Peng.²⁶