Red cell membrane protein analysis. Red cell membranes were separated on 10% Laemmli gels and Coomassie stained or immunoblotted using antibodies as shown. Loading: C1, C2, controls 1 and 2. P indicates proband (Stockport A-II-1). The RhAG is heavily glycosylated (seen as a broad 50- to 80-kDa band). In the proband, the amount of RhAG was reduced and the band was slightly shifted, suggesting hyperglycosylation. The Rh polypeptides were also reduced in expression, in proportion to the reduction of RhAG. CD47 appears unchanged. The reduction in stomatin has been shown previously and is characteristic of OHSt.