15(S)-HETE–induced IL-8 promoter-driven luciferase reporter-gene expression requires STAT binding site. (A) Sequence of a 1.5-kb cloned human IL-8 promoter region showing the STAT binding site. (B) HRMVECs were transfected with either empty vector or IL-8 promoter-luciferase constructs, quiesced, and treated with and without 0.1 μM 15(S)-HETE for 6 hours, and cell extracts were prepared and analyzed for luciferase activity. *P < .01 versus pGL3-hIL8p-WT control; **P < .01 versus pGL3-hIL8p-WT + 15(S)-HETE. pGL3-hIL8p-WT, wild-type promoter construct; pGL3-hIL8p-M, mutant promoter construct. Sequence in bold represents STAT binding site; +1 indicates transcription start site.