15(S)-HETE–induced IL-8 expression requires activation of Jak2-STAT-5B signaling in HRMVECs. (A,B) HRMVECs were transduced with Ad-GFP, Ad-dnJak2, or Ad-dnSTAT-5B with 80 MOI, quiesced, and treated with and without 0.1 μM 15(S)-HETE for 4 hours, and RNA was isolated and analyzed for IL-8 mRNA levels by RT-PCR. (C) All the conditions were the same as in panels A and B, except that medium was collected and tested for IL-8 release by ELISA. The bar graphs in panels A through C represent the mean ± SD values of 3 independent experiments. *P < .01 versus Ad-GFP; **P < .01 versus Ad-GFP + 15(S)-HETE.