Blockade of Jak2 activation inhibits 15(S)-HETE–stimulated STAT-5B phosphorylation. HRMVECs were transduced with Ad-GFP or Ad-dnJak2 at 80 MOI, quiesced, and treated with and without 15(S)-HETE (0.1 μM) for the indicated time periods, and cell extracts were prepared and analyzed by Western blotting for tyrosine phosphorylation of STAT-5B, as described in Figure 2A legend (top panel). The blot was reprobed with anti–STAT-5B antibodies for normalization (middle panel). The same samples were analyzed by Western blotting using anti-Jak2 antibodies to show the overexpression of dnJak2 (bottom panel).