Figure 8
![Figure 8. Thrombin generation in the plasma of lactadherin-deficient mice and control littermates. The reaction mixture consisted of plasma (80 μL) and the trigger solution (3 ng purified Russell viper venom in 40 μL), fluorogenic thrombin substrate Z-Gly-Gly-Arg-AMC (2.5 mM), and CaCl2 (10 mM). The generation of thrombin was measured as a function of fluorescence in a fluorimeter over a period of 30 minutes. Thrombin was then calculated using an α2-macroglobulin thrombin complex standard as described by Hemker et al.27 Representative data are presented. Blood samples from 5 mice from each group (lactadherin−/− and littermate controls [lactadherin+/+]) were pooled for each experiment.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/113/6/10.1182_blood-2008-07-167148/4/zh80060930840008.jpeg?Expires=1722823970&Signature=fkIDMOdKMj2c4cangc3qJaT8fqTpAegbOVpQUy2TRWg5lqVxW35MAWPVg9~PRf0TcQw8X-YfLLfSZgjTyRtjgVUJpNKPJzngbR5MpVd-SYWzUpOfwll6Ntj1FxwxZorWemPJV40VWFV-m6RHyxLI7no5OSfYgi0rkR9w3waxwS3BWocUYqBThj90tBsxgWm81QWZNIQWtYDmrxGti2TtoWuEErug579gaaFV13nGAVFpxICWA2apdDieQdMeY6xFESjfiFnWRMvx7GMHg5T0wQ-W5yfAuYIDraBy8l-flwVhOGlr2gRnYj7U5661PmSiXb8BOKNOzEjVa~qBsic-sA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Thrombin generation in the plasma of lactadherin-deficient mice and control littermates. The reaction mixture consisted of plasma (80 μL) and the trigger solution (3 ng purified Russell viper venom in 40 μL), fluorogenic thrombin substrate Z-Gly-Gly-Arg-AMC (2.5 mM), and CaCl2 (10 mM). The generation of thrombin was measured as a function of fluorescence in a fluorimeter over a period of 30 minutes. Thrombin was then calculated using an α2-macroglobulin thrombin complex standard as described by Hemker et al.27 Representative data are presented. Blood samples from 5 mice from each group (lactadherin−/− and littermate controls [lactadherin+/+]) were pooled for each experiment.
