The endogenous production of CXCL12 and CXCL14 is responsible for activin A–induced DC migration. (A) M3 (100 nM) inhibits DC migration in response to activin A, CXCL12, and CXCL14. (B) Effect of anti-CXCR4 (7.5 μg/mL)- and anti-ChemR23 (3 μg/mL)–blocking antibodies on DC migration. (C) Effect of AMD3100, a CXCL12 receptor antagonist, on DC migration. Cells were preincubated for 30 minutes with different concentrations of the inhibitor/mAb and then tested in chemotaxis assays. (D) Cross-desensitization experiments using 100 ng/mL CXCL12, 100 ng/mL CXCL14, 100 ng/mL CCL3, and 3 nM chemerin. Cells were preincubated with the different chemotactic agonists at 37°C for 30 minutes, washed, and tested in the chemotaxis assay. Migration was performed using 100 ng/mL activin A, 100 ng/mL CCL3, 100 ng/mL CXCL12, 100 ng/mL CXCL14, 3 nM chemerin, and 10 nM PMA. **P < .005 by paired Student t test versus respective control groups.