The EGFP-positive cells between the DA and PCV are early hematopoietic cells. (A,B) Lateral views of the trunk of 48 hpf Tg(runx1P2:EGFP)/Tg(gata1:DsRED) double-transgenic embryo confirms the localization of round EGFP-expressing cells between the DA and PCV (arrowheads). (C,E,G,I) Lateral views of the trunk regions of uninjected (uninj) and MO-injected 48 hpf Tg(runx1P2:EGFP) embryos. (D,F,H) Lateral bright-field and (D′,F′,H′) fluorescent images of 48 hpf MO-injected transgenic embryos together with uninjected controls. (D,E) The hematopoietic EGFP-expressing cells are abolished in runx1 runt domain MO-injected embryos (arrowheads). (F,G) Injection of scl MO ablated the EGFP-positive cells between the DA and PCV (arrowheads). (H,I) Injection of gata1 MO increased EGFP expression in the AGM region compared with controls (arrowheads).