Comparisons of PODXL/CD49f high and low MSCs. (A) PODXL^hi/CD49f^hi MSCs survive longer in lung after intravenous infusion. One million passage 2 MSCs that were from donor 1 and 8 were infused intravenously into mice with MI. One day after the infusions, DNA was extracted for real-time PCR of Alu sequences and RNA was extracted for real-time RT-PCR for human GAPDH mRNA. Values are expressed as % values obtained with 1 million freshly isolated MSCs that were either PODXL^hi/CD49f^hi or PODXL^lo/CD49f^lo. *P < .05 by Student t test (n = 5). (B) Immunocytochemistry demonstrating that PODXL^hi/CD49f^hi MSCs express greater levels of ezrin. The MSCs were from passage 2 of donor 8. (C) Western blot assays demonstrating that PODXL^hi/CD49f^hi MSCs express greater levels of phosphorylated Akt (p-Akt) and p44/42 MAPK. The MSCs were passage 2 from donor 8. (D) Tissue distributions of intravenously infused MSCs. One million passage 2 MSCs (donor 1 and donor 8) that were either PODXL^hi/CD49f^hi or PODXL^lo/CD49f^lo were infused intravenously into control mice or mice with MI, and tissues were recovered 1 day after the MSC infusions. Values from real-time PCR assays for human Alu sequences are expressed either as percent of infused human cells or human cell numbers. Error bars indicate range of values. *P < .05 versus PODXL^hi/CD49f^hi in control mice and P < .05 versus PODXL^lo/CD49f^lo in MI mice by nonparametric Mann-Whitney U test. **P <.05 versus PODXL^hi/CD49f^hi in control mice and P < .05 versus PODXL^lo/CD49f^lo in MI mice by 2-tailed Student t test. †P < .05 versus PODXL^lo/CD49f^lo in MI mice by 2-tailed Student t test. (E) Terminal deoxynucleotidyl transferase dUTP nick end labeling assay of heart from mice 2 days after MI induction indicating apoptotic cells in region of infarction.