Coagulation parameters after liver-directed AAV2-mediated FIX gene therapy in hemophilia B dogs. The ACT (A), WBCT (B), aPTT (C), FIX activity levels (D), and FIX antigen concentration (E) as a function of time after AAV2/cFIX vector administration in hemophilia B dogs, Brad (●) and Semillon (). Normal ranges of 1 to 2 minutes for ACT, 6 to 10 minutes for WBCT, and 8.0 to 14.4 seconds for aPTT are indicated by the shaded bars.