Figure 2
Figure 2. A fraction of recipient mice that received transplants of total bone marrow cells expressing oncogenic Kras from its endogenous locus developed T-cell leukemia. Flow cytometric analysis of peripheral blood (PB), bone marrow (BM), and spleen (SP) cells isolated from control mice or mice that received transplants of bone marrow cells expressing oncogenic Kras G12D from its endogenous locus. These density plots were gated on live nucleated cells based on forward scatter and propidium iodide staining profiles. More than 98% of cells analyzed here are donor-derived as judged by their CD45.2 expression. Representative data are shown. The percentages of cells in quadrants of interest are indicated.

A fraction of recipient mice that received transplants of total bone marrow cells expressing oncogenic Kras from its endogenous locus developed T-cell leukemia. Flow cytometric analysis of peripheral blood (PB), bone marrow (BM), and spleen (SP) cells isolated from control mice or mice that received transplants of bone marrow cells expressing oncogenic Kras G12D from its endogenous locus. These density plots were gated on live nucleated cells based on forward scatter and propidium iodide staining profiles. More than 98% of cells analyzed here are donor-derived as judged by their CD45.2 expression. Representative data are shown. The percentages of cells in quadrants of interest are indicated.

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