Single cell trajectories of wild-type (WT) and Cdc42-deficient DCs migrating along a CCL19 gradient in 2-dimensional (top panel) and 3-dimensional (bottom panel) settings. Despite reduced directionality, Cdc42-deficient DCs were still able to migrate with residual efficiency on 2-dimensional surfaces. In contrast, lack of Cdc42 dramatically reduced cell displacement in 3-dimensional environments due to irreversible cell entangling. See the complete figure in the article beginning on page 5703.