Characterization of AE9a in leukemogenesis. (A) The structure of the AE, AEtr, AEtr-L148D, AE9a, AE9a-R174Q, AE9a-delNHR1, AE9a-delNHR2, and AE9a-del(350-428). Triangles point to the location of 2 mutations in the RHD domain. (B) The expression of different AE mutant proteins. (C) The Kaplan-Meier survival curves of mice transplanted with hematopoietic cells expressing AEtr and AEtr-L148D (top panel) or AE9a and AE9a-R174Q (bottom panel). (D) Kaplan-Meier survival curves of mice transplanted with hematopoietic cells expressing AE9a or its mutant forms. At least 2 independent sets of experiments were performed with each group of transplantations.