Roles of β2-integrins, P-selectin, and platelets in anti-MPO–induced glomerular leukocyte adhesion in mice treated with high-dose anti-MPO alone. Mice underwent treatment with high-dose (200 μg) anti-MPO in combination with various inhibitory antibodies administered prior to anti-MPO. (A) Effect of mAbs against either β2-integrins/CD18 (n = 6) or P-selectin (n = 5) on glomerular leukocyte adhesion induced by high-dose anti-MPO. Also shown are results from mice treated with anti-MPO alone. (B) Effect of platelet depletion on glomerular leukocyte adhesion induced by high-dose anti-MPO. Mice received antiplatelet serum 4 hours prior to undergoing treatment with anti-MPO (n = 6). These experiments were compared with results from mice pretreated with nonspecific rabbit IgG (n = 6).