Effect of high-dose anti-MPO on leukocyte–endothelial cell interactions in glomerular capillaries and cremasteric postcapillary venules. (A) Intravital microscopic assessment of glomerular leukocyte adhesion in mice treated with high-dose (200 μg, intravenously) anti-MPO (n = 8) versus anti-OVA (n = 10). A basal assessment of glomerular adhesion was performed, anti-MPO or anti-OVA was administered, then recordings of glomerular adhesion were performed 15, 30, and 60 minutes later. (B,C) Leukocyte rolling flux (B) and adhesion (C) in cremasteric postcapillary venules of mice treated with high-dose anti-MPO (n = 5) or anti-OVA (n = 4). Following assessment of basal leukocyte rolling and adhesion, mice received 200 μg anti-MPO or anti-OVA intravenously, and rolling and adhesion were reassessed over the subsequent 60 minutes. (*P < .05; **P < .01 vs the anti-OVA group at the corresponding time points.)