Sixty-minute LPS treatment is sufficient to promote anti-MPO–induced glomerular leukocyte adhesion. (A) Effect of anti-MPO or anti-OVA in otherwise untreated mice. An initial reading of glomerular adhesion was made; then mice received either anti-MPO or anti-OVA (n = 6-7/gp, 50 μg, intravenously), and adhesion was reassessed 15, 30, and 60 minutes later. (B) Assessment of the ability of anti-MPO to induce a glomerular adhesion response 60 minutes after LPS (0.1 μg) administration. Mice were treated in an identical fashion to Figure 1, except anti-MPO or anti-OVA (n = 6/gp, 50 μg, intravenously) was administered 1 hour after LPS. (*P < .05; **P < .01 vs anti-OVA at the corresponding time points.)