DNMT3A and 3B are targets of miR-29b. (A) Schema of the 2 firefly luciferase reporter constructs for DNMT3A and 3B, indicating the interaction sites between miR-29b and the 3′ UTRs of the DNMT3s. (B) Dual luciferase assay of K562 cells cotransfected with firefly luciferase constructs containing the DNMT3A or 3B wild-type or mutated 3′ UTRs and pre-miR-29b or scrambled oligonucleotides as indicated. The firefly luciferase activity was normalized to Renilla luciferase activity. The data are shown as relative luciferase activity of pre-miR-29b transfected cells with respect to the control (scrambled oligonucleotide) of a total of 9 experiments from 3 independent transfections. Bars represent SD. (C) Dual luciferase assay of K562 cells cotransfected with firefly luciferase constructs containing the DNMT1 3′ UTR and pre-miR-29b or scrambled oligonucleotides. The data are shown as relative luciferase activity of pre-miR-29b–transfected cells with respect to the control (scrambled oligonucleotide) of 9 experiments obtained from 3 independent transfections. Bars represent SD.