Cell-signaling intermediaries in eFLCs. Shown are Western blot analyses for (A) phospho-STAT5 (pSTAT5) with β-actin as a loading control, cultured as indicated, and (B) phospho-p42/44 (pp42/44), and unphosphorylated p42/44 as a loading control (reprobed), cultured as indicated. Both panels A and B were induced for 10 minutes, after overnight serum starvation. (C) Phospho-p38 (pp38), after 24 and 32 hours in culture, plus β-actin loading control at 32 hours, run in parallel.