Tumor protection in WT and βc knockout mice vaccinated with different cytokine-producing tumor cells. (A) Survival of C57BL/6 mice vaccinated subcutaneously with irradiated cytokine-producing B16 cells. Mice were vaccinated with 106 B16 cells secreting GM-CSF, FLT3-L, or IL-3 and challenged 1 week later with 5 × 105 viable unmodified B16 cells. The graph is representative of 3 independent experiments. (B) Cytokine production by the modified B16 tumor cell lines used in the experiments. Cytokine-producing B16 cells were irradiated and seeded in triplicate at a density of 106 cells per well. Supernatant of cells was collected after 24 hours and IL-2, IL-4, IL-5, IL-6, IL-10, IL12p70, MCP-1, IFN-γ, and TNF-α were measured using inflammation and Th1/Th2 CBA kits. The positive and negative controls represent the DMEM complete medium and the 1250 pg/mL CBA standard curve, respectively. Each plot is representative of 1 single cell supernatant and illustrates 1 of the 2 independent experiments.
