Ex vivo polyfunctional profiles of HIV-specific CD8⁺ T-cell populations. Polyfunctional profiling of representative HLA B*2705-restricted KK10 (A)– or HLA A*0301-restricted RY10 (B)–specific CD8⁺ T-cell populations (from 2 patients of 5 in each case) with different antigen sensitivities along a peptide concentration gradient. Antigen sensitivity of these populations was determined in standard intracellular cytokine-staining assays for IFN-γ secretion conducted directly ex vivo.