miR-184 negatively effects NFAT1 protein synthesis. (A) Quantification and representative blot of NFAT1 protein expression in UCB CD4⁺ T cells 16 hours after transfection with antisense to miR-184 (n = 3). (B) qRT-PCR analysis of samples in (A), confirming no significant change in NFATc2 mRNA quantity. (C) Quantification and representative blot of NFAT1 protein expression in AB CD4⁺ T cells under the following transfection with pre-miR-184 (n = 4). Blot bands were quantified using ImageJ software. (D) Western blot analysis of NFAT1 protein expression in AB CD4⁺ T cells 16 hours after transfection with antisense to miR-184.