Expression and improved migration of CCR4⁺ T cells established from persons affected by HL. (A) The expression of CCR4 by CTR () and transduced (■) T cells established from 4 persons with HL. The data are mean ± SD. (B) A representative phenotypic analysis. (C) The migration of CTR () and CCR4⁺ (■) T cells toward TARC gradients, using a transwell migration assay. T-cell migration was evaluated using culture supernatants collected from HDLM-2 and L428, which physiologically produce high amounts of TARC, and against Karpas genetically modified to produce TARC (K/TARC). K/wt represents migration toward Karpas wild-type, which produces a negligible amount of TARC, as a negative control. Migration is significantly increased for CCR4⁺ T cells compared with CTR T cells. In addition, the figure shows that the improved migration of CCR4⁺ T cells (■) is TARC mediated as it is inhibited by addition of anti-TARC antibodies but not by addition of an isotype control.